Figure 1

Real-Time RT-PCR amplification plot of β-actin mRNA. Representative RT-PCR plot resulting from the amplification of β-actin cDNA templates (1/20 volume) synthesized from 1 μg of total RNA (see Materials and Methods for details). For each time point, the cDNA was generated and quantified from three independent experiments and run in triplicates. The calculated cycle threshold (Ct = 99) provides an arbitrary cut off point at which a Ct value is assigned for each sample.