Fig. 3

hnRNPK promotes the transcription of SRSF1 by binding to its promotor. a Schematic diagram showing the two putative hnRNPK binding motifs within the promoter region of human SRSF1 loci. b Quantitative ChIP-PCR (ChIP-qPCR) was performed using hnRNPK antibody in GC-803 cells. The negative IP was performed using anti-rabbit IgG. c Relative expression of hnRNPK (upper) and SRSF1 (lower) expression in MGC-803 cells after transfection with si_control or si_hnRNPK, pcDNA or pcDNA-HNRNPK. d The relative luciferase activities in MGC-803 cells after transfected with pcDNA or pcDNA-hnRNPK. The cells were co-transfected with either a wild type pGL-3-promoter construct (WT) or a mutant promoter (MUT) of SRSF1. For all quantitative results, the data are presented as the mean ± SEM, and the error bars represent the standard deviation obtained from three independent experiments. *p < 0.05; **p < 0.01