Fig. 4

CDX2 or let-7b over-expression and COL11A1 silencing inhibits breast cancer growth and metastasis. a Tumor growth curve of nude mice transfected with over-expressed CDX2, let-7b mimic and shRNA against COL11A1 on the 7th, 14th, 21st and 28th day after injection; On the 28th day, the nude mice in each group were euthanized, and the tumors were taken. b Representative images of xenograft tumors in nude mice with various transfections; c average weight of xenograft tumors; d lymph node metastasis positive rate in different operations; e representative images of HE staining of metastatic nodules in the livers (upper) and lungs (lower) (×400); f relative protein expression of CDX2, COL11A1, E-cadherin and Vimentin determined by western blot analysis. *p < 0.05 vs. NC of CDX2 over-expression, NC of shRNA targeting COL11A1 or NC of let-7b mimic (n = 8). All the data in this experiment were measurement data and described as mean ± standard deviation. Unpaired t-test was applied for analyses of c, d and f. In b repeated ‘measures ANOVA was conducted and followed by Bonferroni post hoc test