Fig. 6
CDC42 was required for miR-149-mediated progression of NB. a The expression of CDC42 protein was measured in SK-N-BE(2)C and SK-N-SH cells transfected with miR-NC, miR-149, miR-149 + pcDNA or miR-149 + CDC42 by western blot. The IC50 of Dox (b) and cell proliferation (c) was analyzed in SK-N-BE(2)C and SK-N-SH cells transfected with miR-NC, miR-149, miR-149 + pcDNA or miR-149 + CDC42 by MTT. d Colony formation was measured in SK-N-BE(2)C and SK-N-SH cells transfected with miR-NC, miR-149, miR-149 + pcDNA or miR-149 + CDC42. e Cell apoptosis was detected in SK-N-BE(2)C and SK-N-SH cells transfected with miR-NC, miR-149, miR-149 + pcDNA or miR-149 + CDC42 by flow cytometry. *P < 0.05, **P < 0.01, compared with miR-NC or miR-149 + pcDNA group