Fig. 6

Overexpression of DLX2 could reverse the miR-216b-5p effects on proliferation, apoptosis, migration and invasion in Hep3B and Huh7 cells. Hep3B and Huh7 cells were transfected with control, miR-NC, miR-216b-5p, miR-216b-5p + pcDNA or miR-216b-5p + DLX2. a–d The cell proliferation and apoptosis were detected by MTT and Flow cytometry. e, f The protein levels of PARP, cleaved PARP and cleaved caspase-3 were measured by western blot. g–j The migration and invasion of transfected Hep3B and Huh7 cells were detected through Transwell assay. k, l The protein levels of E-cadherin, N-cadherin and Vimentin were assesses by western blot. *P < 0.05