Fig. 2

LIFR-AS1 sponges miR-942-5p to upregulate ZNF471. a, b RIP assays performed with anti-Ago2 antibody showed that miR-942-5p and LIFR-AS1 were detected in Ago2 immunoprecipitates from both A549 and H1299 cells. c Overexpression of miR-942-5p decreased the mRNA level of ZNF471 in both A549 and H1299 cells. d Bioinformatic analysis predicted that the wild-type (wt) 3′-UTR of ZNF471 harbored a potential miR-942-5p binding site. A mutated (mut) ZNF471 3′-UTR was constructed by disruption of the miR-942-5p binding site. e Luciferase reporter assays showed that overexpression of miR-942-5p decreased the luciferase activity of the reporter with the wild-type but not mutated ZNF471 3′-UTR. f Ago2 RIP assays were performed in A549 cells transfected with miR-942-5p mimic together with wild-type or mutated ZNF471. Results are expressed as fold enrichment relative to control mimic. g Wild-type but not mutated LIFR-AS1 promoted the mRNA expression of ZNF471, which was reversed by miR-942-5p overexpression. h Western blot analysis of ZNF471 protein levels in A549 cells transfected with indicated constructs. ^*P < 0.05; n.s. indicates no significance