Fig. 1

Hypoxia-enhanced Tie1 expression in human lung cancer cells contributes to reduced cisplatin sensitivity. a and b, Tie1 mRNA levels (a) and protein levels (b) in human pulmonary microvascular endothelial cells (HPMECs) and human NSCLC cell lines were detected by qRT-PCR and western blot, respectively. *P < 0.05, **P < 0.01, ***P < 0.001. Relative protein levels quantified by ImageJ and normalized to β-actin. (n = 3). c Cell viability of A549 cells, and NCI-H1975 cells exposed to hypoxia for 0, 6, 12, 24, and 48 h. d and e, A549 cells, and NCI-H1975 cells were exposed to hypoxia for 0, 6, 12, 24, and 48 h. mRNA levels (d) and protein levels (e) were detected by qRT-PCR and western blotting, respectively. Relative protein levels quantified by ImageJ and normalized to β-actin. *P < 0.05, **P < 0.01, ***P < 0.001. (n = 3). f A549 cells and NCI-H1975 cells stably transfected by scramble or Tie1-shRNA lentiviruses were incubated under hypoxic conditions for 48 h. Proteins were analyzed using western blotting. Relative protein levels quantified by ImageJ and normalized to β-actin. **P < 0.01, ***P < 0.001. (n = 3). g A549 cells and NCI-H1975 cells stably transfected by scramble or Tie1-shRNA lentiviruses were treated with cisplatin under normoxic or hypoxic conditions for 48 h. Cell viability was assessed by CCK-8 assays. *P < 0.05, **P < 0.01, ***P < 0.001 vs Normoxia + sh-Scr, ^#P < 0.05, ^##P < 0.01 vs Hypoxia + sh-Scr, using two-way ANOVA followed by Bonferroni post hoc test. (n = 3)