Fig. 7

The expression profile of drug resistance genes in HT-29 and Caco-2 derived spheroids compared to their adherent counterparts. The comparative real-time PCR analysis of ABC transporter genes; ABCB1, ABCC1 and ABCG2 in generated spheroids and their parental cells showed a the higher expression of ABCB1 and ABCG2 in HT-29 derived spheroids than HT-29 parental cells, the expression of ABCC1 was also increased, although this increase was not significant (p-value = 0.14), b whereas there was not observed significant differences between Caco-2 spheroids and their parental cells. Data are presented as mean ± SD from four independent experiments as * = P < 0.05, ns = not significant