Fig. 1

Overexpression of DPY30 is correlated with EMT and tumor metastasis in human colorectal carcinoma. A Analysis of DPY30 expression profile across tumor samples and paired healthy tissues using the GEPIA online database, and the tissue-wise expression of DPY30 in different cancer types using The Human Protein Atlas website. B DPY30 protein expression in the paired tissue samples was detected by western blot (N: distal healthy tissues. P: para-cancerous tissues. T: CRC tumor tissues). Comparison of the relative protein expression levels of DPY30, compared with N and P (n = 15). Data are expressed as mean ± SEM. ***P < 0.001. C Expression levels of DPY30, E-Cadherin, N-Cadherin, and Vimentin in tumor in situ and para-tissue by immunohistochemical staining analysis. D Correlation scatter plot of the IHC score of DPY30 and the EMT markers E-Cadherin, N-Cadherin, and Vimentin, respectively; (n = 15). E Representative immunostaining images of DPY30 in a CRC tissue microarray from patients with or without positive lymph nodes (Number of patients = 94), along with attached scores displaying the correlation of DPY30 immunostaining intensity with the number of positive lymph nodes. Data are presented as mean ± SEM. *P < 0.05