Fig. 2
From: DPY30 promotes colorectal carcinoma metastasis by upregulating ZEB1 transcriptional expression
Knockdown of DPY30 inhibits EMT in CRC cells. A Western blotting analysis of DPY30 and EMT markers in CRC cells RKO, SW620, HCT116, SW1116, HT29, SW480, Caco2, KM12C, and the normal colonic epithelial cell line NCM460 (left). Densitometry quantifications were performed using Image Lab software (right). B Quantitative real-time PCR analysis of the mRNA levels of DPY30 and EMT in CRC cells. C Western blotting (left) and qPCR (right) analysis of DPY30 and EMT markers in DPY30-knockdown CRC cell lines. Data are expressed as mean ± SEM of three independent experiments. *P < 0.05, ***P < 0.001. D Representative phase-contrast images of HT29 and SW480 cells showing morphological alterations following DPY30 knockdown