Table 1 Primers used for gene expression detection
From: DPY30 promotes colorectal carcinoma metastasis by upregulating ZEB1 transcriptional expression
Gene | Primer forward (5ʹ–3ʹ) | Primer reverse (5ʹ–3ʹ) |
|---|---|---|
RT-qPCR primers | ||
 DPY30 | AACGCAGGTTGCAGAAAATCCT | TCTGATCCAGGTAGGCACGAG |
 E-Cadherin | TGAAGGTGACAGAGCCTCTGGAT | TGGGTGAATTCGGGCTTGTT |
 N-Cadherin | AGATAGCCCGGTTTCATTTGAG | ATGTTGGGTGAAGGGGTGCT |
 Vimentin | AATGGCTCGTCACCTTCGTG | CAGATTAGTTTCCCTCAGGTTCAG |
 Fibronectin | CACTTCAGTGGGAGACCTCGAG | GGTCCCTCGGAACATCAGAAAC |
 Snail1 | GCTACTGCTGCGCGAATCG | GTAGGGCTGCTGGAAGGTAAACT |
 Slug | TTCGGACCCACACATTACCTTG | ACCTGTCTGCAAATGCTCTGTTG |
 Twist1 | CGGCTCAGCTACGCCTTCT | CAATGACATCTAGGTCTCCGGC |
 Twist2 | CATGTCCGCCTCCCACTAGC | ATGTGCTCACTCCCGCCAAC |
 Zeb1 | AGGCTATAAACGCTTTACCTCTCTG | TTACGATTACACCCAGACTGCGT |
 β-actin | CATGTACGTTGCTATCCAGGC | CTCCTTAATGTCACGCACGAT |
ChIP-qPCR primers | ||
 #1 Zeb1 | AAATTCAGCAGTGCCCACG | TTACGACACTCCCGGCTTTAC |
 #2 Zeb1 | GGCAAAGTGGAGTGGGAAAG | AGACATAACGGTTCAGGGAGA |
 #3 Zeb1 | TCTTACCTGGTCTCTCTCCGC | AGAAAAGTGGCCAGTGCGT |