Fig. 1
ET-1 and its receptors are expressed in GBC cells. a Kaplan–Meier post-diagnosis overall-survival (OS) estimation of GBC patients, according to ET-1 receptors expression. Each graph shows the number of patients in each group (n), the median OS time in months, and overall survival rate (%). p-values were calculated using a log-rank (Mantel–Cox) test. b ET-1 receptors, ETAR (EDNRA) and ETBR (EDNRB), transcripts levels were measured by RT-qPCR in four GBC cell lines. c ET-1 receptors ETAR and ETBR, ECE1 and NEP protein levels were detected by western blot in four GBC cell lines. Representative images from three independent experiments are shown. d ET-1 transcripts (EDN1) levels were measured by RT-qPCR in four GBC cell lines. e ET-1 extracellular levels (pg/ml) were measured by ELISA in CAVE-1 and NOZ GBC cells. Data represent averages ± SEMs (n = 3). ANOVA and Student’s tests were used. *p < 0.05