Fig. 5
ET-1 signaling blockage with macitentan sensitizes GBC cells to gemcitabine. a ECE1, ETAR and ETBR protein levels were detected by western blot in NOZ cells treated with 14 µM GEM and/or 1 µM MAC for 24 h. b Cells were seeded in 96-well plates for 24 h and treated with increased concentrations of gemcitabine (GEM) alone or in combination with 1 μM MAC for 72 h. Cells were incubated with MTS reagent for 2 h and absorbance was measured at 490 nm and plotted as percentage. c Cell viability of NOZ cells treated with 14 µM GEM and/or 1 µM MAC for 72 h measured by crystal violet assay. d ZEB1, β-catenin, NF-kB and cleaved PARP (cl-PARP) detection by western blot in NOZ cells treated with 14 µM GEM and/or 1 µM MAC for 24 h. Data represent averages ± SDs (n = 3). ANOVA test was used. *p < 0.05