Fig. 3

Photomicrographs of liver and spleen tissues. A) Liver tissue: (A) control group showing normal structure; (B) liver cells from B16 WT-injected mice revealing small foci of infiltrative cells (black arrow); (C) liver cells from B16 TPC2 KO-injected mice displaying fibrosis (green arrows), aggregations of infiltrative cells (black arrow), and haemorrhage (H); (D) liver cells from B16 TPC2 KO-injected mice displaying a congested vein with haemorrhage and oedema (green arrow), infiltrative cells (black arrow), and cytoplasmic degeneration (red arrows). (HE-400X). B) Spleen tissue: (A) control spleen showing well-defined splenic structure (WP), white pulp (RP), and red pulp; (B) spleen tissue from B16 WT-injected mice revealing healthy spleen tissue, (WP) white pulp, and (RP) red pulp; (C) spleen tissue from B16 TPC2 KO-injected mice displaying pathological changes with a minimized lymph node centred with hyaline degeneration (H); (D) spleen tissue from B16 TPC2 KO-injected mice presenting an ill-defined structure and numerous large macrophages (black arrows). C) Table showing a significant decrease in the spleen lymph node area and volume in the B16 WT and B16 TPC2 KO groups versus the control