Fig. 1
Antiproliferative effect of IFN-β on different cell lines. a A549 lung adenocarcinoma cells, or b PH5CH non-neoplastic liver cells were kept under 1000 IU/ml IFN-β or mock treated. Cell proliferation was monitored by automated life-cell imaging (IncuCyte S3). Data represents mean ± SD of three biologically independent repetitions. Unpaired student`s t-test. c + d A549IFNR−DKO (H2B-mCherry labelled) and A549NT control cells (cytosolic GFP labelled) were cocultured (initial ratio of 1:9) for 18 days in medium containing 5000 IU/ml IFN-β (or mock) and frequently split. c Representative microscopy images, more in Additional file 1: Fig. S1. d Ratio of A549INFR−DKO to A549NT cells was determined at days 0, 4, 11 and 18 by flow cytometry and normalised to mock condition. Data shows mean ± SD of two separately passaged cocultures