Fig. 2

In vitro selection of IFN-β-insensitive cell populations upon long-term treatment. a Schematic overview over selection process. b After long-term (6 weeks) IFN-β treatment and IFN-free recovery (1 week), cells were stimulated with IFN-β and growth rates were determined relative to mock-treated control (included for simplicity at x = 10⁰). c Absolute growth rates (duplications per hour) of PH5CH^IFN−β and PH5CH^mock unstimulated and stimulated with 8000 IU/ml IFN-β. PH5CH^IFN−β corresponds to three independently passaged long-term selected cell populations, PH5CH^mock to three technical replicates of one population. Data displayed as mean ± SD