Fig. 4

Antiproliferative effects of IFN-β on hepatoma cells. a Hepatoma cells were mock treated or stimulated with increasing doses of IFN-β (1–8000 IU/ml) and cell numbers were monitored for 96 h (imaged every 6 h) by live-cell imaging. Shown are growth rates normalised to untreated controls, for a medium (222 IU/ml) and the highest (8000 IU/ml) dose of IFN-β; see Additional file 4: Fig. S4 for all doses. b Full titration of HLE and HLF (dedifferentiated, high malignity), PLC (well differentiated, low malignity) and the control cell line PH5CH. c Relative growth rates at 8000 IU/ml IFN-β plotted against respective migration rates as determined before (Fig. 3b); plot against invasiveness see Additional file 3: Fig. S3f. Linear regression was performed and Pearson correlation was calculated. All graphs display mean (± SD in a and b) of three independent repetitions