Fig. 1

The schematic diagram of the application of scRNA-seq in ICB. Tumor or peripheral blood mononuclear cells are obtained from animal models or human patients and are first dissociated into single-cell suspensions. The cells are then lysed for reverse transcription and amplification to construct the cDNA library. The resulting scRNA-seq data can be applied to study the dynamic changes in the TME or peripheral blood during ICB therapy, discover novel biomarkers predictive of ICB response, and search for potential therapeutic targets. scRNA-seq, single-cell RNA sequencing; ICB, immune checkpoint blockade. Created with Figdraw (www.figdraw.com)