Fig. 1

Single-cell sequencing data clusters with cell type annotation. A Visualization of all gastric cancer cells using the Uniform Manifold Approximation and Projection (UMAP) function in the R Seurat package (v. 4.0.3). B Gastric cancer cells were divided into the tumor-adjacent normal, primary tumor, and metastasis tumor groups according to their origin tissue. C UMAP plots showing the distribution and abundance of GLUT3 and lactate dehydrogenase A (LDHA) in gastric cancer. D GLUT3 and LDHA expression in tumor-adjacent normal, primary tumor, and metastasis tumor groups quantified using a violin diagram. E Scatter plots showing the correlation between the expression of GLUT3 and lactylation-related pathways. F Gastric cancer cells were divided into low and high GLUT3 expression groups. G Violin diagram showing the expression of GLUT3 and LDHA in the low and high GLUT3 expression groups. H Correlation between GLUT3 and LDHA expression in the primary and metastatic tumor groups