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Fig. 2 | Cancer Cell International

Fig. 2

From: CD56 polysialylation promotes the tumorigenesis and progression via the Hedgehog and Wnt/β-catenin signaling pathways in clear cell renal cell carcinoma

Fig. 2

Knockout of CD56 inhibited proliferation, invasion and migration of ccRCC cells in vitro and in vivo. Real-time qPCR (A) and western blot (B) showed CD56 expression in 786-O cells using CRISPR/Cas9-mediated genome editing. C CCK-8 assay was performed to determine the effect of knockout-CD56 on the proliferation ability. D Knockout of CD56 suppressed the colony formation ability of 786-O cells compared with control cells and scrambled cells. E Knockout of CD56 inhibited the migration ability of 786-O cells by cell scratch assay within 6 h. F Transwell assay showed knockout of CD56 inhibited the 786-O cells invasion ability. G Morphology of subcutaneous tumors in nude mice after injection for four weeks (n = 6). H Average weight of tumor tissue in nude mice (n = 6). All values represented the mean ± SD of three independent experiments. Scale bars: 400 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by one-way ANOVA (A, B, D, E, F), two-way ANOVA (C), or unpaired two-tailed Student’s t test (H)

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Cancer Cell International

ISSN: 1475-2867

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