Fig. 4

CD56 is polysialylated in ccRCC cells. A Primary structure diagram of CD56 and CD56-N459,488Q. The pentagrams represent the N-glycosylation sites. N represents asparagine and Q represents glutamine. B Polysialylation of CD56. 786-O and A-498 cells were lysed and then treated with or without PNGase F and NEU1 for 32 h in 37 ℃. C Co-localization of CD56 and PSA-CD56 in 786-O cells. Cells were stained with anti-CD56 (1:50, red), anti-PSA-CD56 (1:24, green), DAPI (blue) and then subjected to confocal fluorescence microscopy. Overexpression of CD56 and CD56-N459,488Q in Caki-1 cells was analyzed by Real-time qPCR (D) and western blot (E). ***P < 0.001, ****P < 0.0001, N.S. represents no statistical significance by one-way ANOVA (D, E)