Fig. 6
HSPA8 deficiency inhibits tumor cell migration and invasion. (A) Boxplots of the HSPA8 expression between tumor and normal tissues in the TCGA pan-cancer cohorts (* p < 0.05, ** p < 0.01, *** p < 0.001). (B) Heatmap of the HSPA8 expression among different cell types in TISCH database. (C) Heatmap of the prognostic value of HSPA8 in the TCGA pan-cancer cohorts. (D) Survival analyses of HSPA8 using K-M analyses in BC RNA-seq and microarray datasets from bc-GenExMiner database. (E) Western blot assays showing the efficacy of siRNAs targeting HSPA8 in BC cell lines. (F) Transwell migration assays were performed to measure the migration abilities of HSPA8 in BC cell lines. (G) Wound healing assays were performed to measure the migration abilities of HSPA8 in BC cell lines. (H) Boxplots of the number of cells migrated per field and relative healing area (% control) in BC cell lines (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). ACC, adrenocortical carcinoma; BLCA, bladder urothelial carcinoma; BRCA, breast invasive carcinoma; CESC, cervical squamous cell carcinoma and endocervical adenocarcinoma; CHOL, cholangiocarcinoma; COAD, colon adenocarcinoma; DLBC, lymphoid neoplasm diffuse large B-cell lymphoma; ESCA, esophageal carcinoma; GBM, glioblastoma multiforme; HNSC, head and neck squamous cell carcinoma; KICH, kidney chromophobe; KIRC, kidney renal clear cell carcinoma; KIRP, kidney renal papillary cell carcinoma; LAML, acute myeloid leukemia; LGG, brain lower grade glioma; LIHC, liver hepatocellular carcinoma; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; MESO, mesothelioma; OV, ovarian serous cystadenocarcinoma; PAAD, pancreatic adenocarcinoma; PCPG, pheochromocytoma and paraganglioma; PRAD, prostate adenocarcinoma; READ, rectum adenocarcinoma; SARC, sarcoma; SKCM, skin cutaneous melanoma; STAD, stomach adenocarcinoma; TGCT, testicular germ cell tumors; THCA, thyroid carcinoma; THYM, thymoma; UCEC, uterine corpus endometrial carcinoma; UCS, uterine carcinosarcoma; UVM, ocular melanomas. ALL, acute lymphoblastic leukemia; AML, acute myeloid leukemia; KIPAN, pan-kidney cancer; LSCC, laryngeal squamous cell carcinoma; OS, osteosarcoma