Fig. 6

Robinin downregulated PI3k-AKT signal pathway via TLR2 in Mia-PACA2 and PANC-1. (A) The expression levels of TLR2, PI3k-p85α, and p-AKT in Mia-PACA-2 were detected by western blot analysis and normalized to β-actin and AKT, respectively. (B) The expression levels of TLR2, PI3k-p85α, and p-AKT in PANC-1 were detected by western blot analysis and normalized to β-actin and AKT, respectively. *P < 0.05, **P < 0.01, vs. the control group, #P < 0.05, ##P < 0.01, vs. the Robinin co-cultivated with CU-T12-9 group. Measurement data were expressed as mean ± standard derivation; data between PC cell and HPNE were compared by t-test, and measurement data among multiple groups were analyzed by one-way analysis of variance with Tukey’s post hoc test. The experiment was repeated three times