Fig. 1

Screening and characterization of circEYA3. A Flowchart of differentially expressed circular RNA (circRNA) screening of patients with hepatocellular carcinoma (HCC) after implantation of ¹²⁵I seeds. B The heat map of differentially expressed circRNA before and after ¹²⁵I seed implantation. C Schematic showing the origin of circEYA3. Sanger sequencing indicated the existence of circEYA3 and the back-splicing junction sites in exons 2 and 6 of EYA3. D The relative levels of circEYA3 in PLC/PRF/5 cell line and in situ HCC in mice with or without.¹²⁵I irradiation were detected by quantitative real-time PCR (qRT-PCR). E qRT-PCR was performed to detect the circEYA3 and EYA3 mRNA levels after RNase R treatment in PLC/PRF/5 and HepG2 cells, respectively. F Fluorescence in situ hybridization (FISH) was carried out to determine the distribution of circEYA3 in HCC cells. Red fluorescence (Cy3-labeled probe) indicates circEYA3, whereas the nuclei were stained with DAPI (blue). Scale bar: 20 μm. G Nuclear-cytoplasmic fractionation experiments were conducted to determine the subcellular localization of circEYA3