Fig. 2

circEYA3 alleviates ¹²⁵I-induced tumor growth inhibition in vivo and DNA damage of HCC in vitro. A qRT-PCR was employed to detect the relative levels of circEYA3 in PLC/PRF/5 and HepG2 cells after transfection with the circEYA3 overexpression plasmid or the control plasmid. B, C Image and bar chart of tumors in mice that received or did not receive ¹²⁵I brachytherapy. D, E, F Immunofluorescence experiments were conducted to observe γH2A.X foci in PLC/PRF/5 and HepG2 cells with or without ¹²⁵I treatment. G, H, I Single cell gel electrophoresis assay (SCGE) or comet assays were performed to observe the DNA damage caused by.¹²⁵I irradiation with or without overexpression of circEYA3