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Fig. 4 | Cancer Cell International

Fig. 4

From: Circular RNA circEYA3 promotes the radiation resistance of hepatocellular carcinoma via the IGF2BP2/DTX3L axis

Fig. 4

The interaction between circEYA3 and insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) and the function of IGF2BP2 in radiation resistance of HCC. A, B RNA pulldown assays revealed the interaction between circEYA3 and IGF2BP2 through mass spectrometry and western blotting (WB). C RNA immunoprecipitation (RIP) assays showed the association between IGF2BP2 and circEYA3. Immunoglobulin G was used as the negative control. D, E qRT-PCR and WB assays were used to validate the efficiency of overexpression and knockdown of IGF2BP2. F–H γH2A.X immunofluorescence assay was performed to reveal the radiation resistance effect of IGF2BP2. I CCK-8 assays showed that the knockdown of IGF2BP2 increased the radiosensitivity of PLC/PRF/5 and HepG2 cell lines

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Cancer Cell International

ISSN: 1475-2867

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