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Fig. 5 | Cancer Cell International

Fig. 5

From: Circular RNA circEYA3 promotes the radiation resistance of hepatocellular carcinoma via the IGF2BP2/DTX3L axis

Fig. 5

CircEYA3 stabilized DTX3L mRNA by interacting with IGF2BP2, thereby increasing the radioresistance of HCC. A-C γH2A.X foci were analyzed in circEYA3-overexpressed HCC cells after transfection with short hairpin RNA (sh)-IGF2BP2 or sh-control plasmids. D, E CCK-8 assays indicated that the radioresistance effect of circEYA3 was rescued by knockdown of IGF2BP2. F Downstream mRNA screening process. G The volcano map of differentially expressed genes in circEYA3-overexpressed (OE) and Vector-OE cells. H Venn diagram showing IGF2BP2-binding mRNA as predicted by starBase/The Encyclopedia of RNA Interactomes database in differentially expressed genes. I Relative expression of five candidates after overexpression of circEYA3 with irradiation was detected through qRT-PCR. J RIP assay showed that the overexpression of circEYA3 promoted the interaction between IGF2BP2 and DTX3L mRNA, but not FGF2 mRNA. K, L The expression levels were measured in circEYA3-overexpressed HCC cells after transfection with sh-IGF2BP2 or sh-control plasmids and irradiation using qRT-PCR and WB

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Cancer Cell International

ISSN: 1475-2867

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