Fig. 4

SEB-stimulated CD8+ 4-1BBζ CAR T-cells produce more IFNγ compared to untransduced T-cells. A Intracellular IFNγ production 24 h after SEB stimulation in untransduced, Mutζ, CD28ζ and 4-1BBζ CD8+ CAR T-cell products in a representative donor. B, C Box plot of intracellular IFNγ-production in B CD4+, and C CD8+ CAR T-cell products. D Degranulation measured by surface CD107a expression 4 h after SEB stimulation in untransduced, Mutζ, CD28ζ and 4-1BB CD8+ CAR T-cell products in a representative donor. E, F Box plot of CD107a expression in E CD4+, and F CD8+ cell products. Experiments were performed with n = 3 independent donors, each on different days; each donor is distinguished using a unique symbol. The mean and SEM are indicated. Comparisons were made between all cell products by one-way ANOVA with Tukey’s correction for multiple comparisons; only statistically significant differences are indicated (*p < 0.05)