Skip to main content
Fig. 5 | Cancer Cell International

Fig. 5

From: The 4-1BBζ costimulatory domain in chimeric antigen receptors enhances CD8+ T-cell functionality following T-cell receptor stimulation

Fig. 5

CD69 activation is increased in CD8+ 4-1BBζ CAR T-cells. A, B Bar graphs of the percentage of CD69 + cells in resting A CD4+, and B CD8+ cell products. C Flow plots of CD69 expression 24 h after SEB stimulation in untransduced, Mutζ, CD28ζ and 4-1BBζ CD8+ CAR T-cell products in a representative donor. D, E Bar graphs of the percentage of CD69+ cells in D CD4+, and E CD8+ CAR T-cell products after 24-h stimulation with SEB. F, G The change in CD69 expression measured by the change in MFI-PE in F CD4+, and G CD8+ cell products after 24-h stimulation with SEB. (H-I) Bar graphs of the percentage of CD69-positive in H CD4+, and I CD8+ CAR T-cell products after 24-h stimulation with PMA/Ionomycin. Experiments were performed with n = 3 independent donors, each on different days; each donor is distinguished using a unique symbol. The mean and SEM are indicated. Comparisons were made between all cell products by one-way ANOVA with Tukey’s correction for multiple comparisons; only statistically significant differences are indicated (*p < 0.05, **p < 0.01, ***p < 0.001)

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us