Table 1 Summary of all antibodies used in the study
Target | Use | Clone | Fluorochrome | Working concentration (µg/mL) | Supplier | Catalogue |
|---|---|---|---|---|---|---|
CD3 | IF | UCT1 | BB515 | 1 | BD Horizon | 564465 |
CD4 | FC | RPA-T4 | BV510 | 1 | Biolegend | 300546 |
CD8 | FC | RPA-T8 | BUV395 | 0.5 | BD Horizon | 563795 |
CD45RO | FC | UCHL1 | BB515 | 2 | BD Horizon | 564529 |
CCR7 | FC | G043h7 | PE-Cy7 | 0.4 | Biolegend | 353226 |
PD1 | FC | EbioJ105 | PerCP-EF710 | 0.5 | Invitrogen eBioscience | 46-2799-42 |
Tim3 | FC | 7D3 | PE | 0.4 | BD Pharmingen | 563422 |
ZAP70 | FC | 1E7.2 | PE | 1 | BD | 344635 |
Mouse IgG1 Kappa Isotype Control | FC | MOPC-21 | PE | As appropriate | BD Pharmingen | 554680 |
Phospho ZAP70 (Tyr 493) | FC | A16043E | PE | 0.05 | Biolegend | 396004 |
CD3ζ | FC | 6B10.2 | APC | 2 | Invitrogen eBioscience | 396004 |
CD69 | FC | REA824 | PE | 0.25 | Miltenyi | 130-112-613 |
Human IgG1 REA Isotype Control | FC | REA293 | PE | As appropriate | Miltenyi | 130-113-462 |
CD107a | FC | REA792 | PE | 0.3 | Miltenyi | 130-111-621 |
Mesothelin | WB | MN1 | – | 1 | Rocklands | 200301A88 |
GAPDH | WB | 6C5 | – | 0.4 | Abcam | Ab8245 |
CD28 | Stim | L295 | – | 1 | BD | 340975 |
MSLN | FC | FAB32652P | PE | 1 | R&D | FAB32652P |
Rat IgG2a Isotype Control | FC | 54447 | PE | 1 | R&D | IC0006P |