Fig. 3
From: PDGF-BB accelerates TSCC via fibroblast lactates limiting miR-26a-5p and boosting mitophagy
JC-1 staining revealed that serum-free incubation of hOMFs decreased the mitochondrial membrane potential (increased green fluorescence and decreased red fluorescence), while PDGF-BB had a protective effect (A). PDGF-BB also increased the proportion of MDC staining in cells and promoted autophagy (C, D). Transmission electron microscopy (TEM) revealed that control cells exhibited a normal tubular mitochondrial network (indicated by yellow arrows), whereas in Star-treated cells, deformed mitochondria were taken up by autophagosomes (indicated by red arrows). Furthermore, PDGF-BB increased the number of autophagic vesicles containing damaged mitochondria and repaired damaged mitochondria in Star-treated hOMFs (B). Fluorescence co-localization demonstrated that PDGF-BB promoted the binding of autophagic mitochondria and lysosomes (E, F). Western blotting showed that the treatment of hOMFs with Star promoted the protein expression of LC3-II, whereas PDGF-BB treatment significantly enhanced the protein expression of LC3-II, PINK1, and Parkin, and decreased the protein expression of LC3-I and P62 (G, H)