Fig. 5
From: PDGF-BB accelerates TSCC via fibroblast lactates limiting miR-26a-5p and boosting mitophagy
CAFs and NFs were isolated from tongue squamous cell carcinoma and adjacent tissues, respectively (A). Immunofluorescence staining revealed that both cell types were fibroblasts, with CAFs in cancer tissues showing high expression of α-SMA and FAP markers (magnification, 200×; scale bar, 20 μm) (B, C). Western blotting demonstrated that PDGF-BB treatment significantly enhanced the protein expression of LC3-II, AMPK, PINK1, and Parkin and decreased the expression of LC3-I and P62 in primary NFs and CAFs (D, E). Immunofluorescence staining showed that CAFs demonstrated higher levels of autophagy than NFs, and PDGF-BB treatment could enhance this effect (magnification, 200×; scale bar, 20 μm) (F–I)