Fig. 6

miRNA microarray analysis revealed a significant downregulation of miR-26a-5p expression (A). qRT-PCR indicated that PDGF-BB treatment downregulated miR-26a-5p expression in normal fibroblasts (B), primary cultured paraneoplastic (C), and cancer-associated fibroblasts (D). Dual luciferase reporter analysis confirmed ULK1 as its target gene (F). Immunofluorescence staining demonstrated that overexpression of miR-26a-5p inhibited PDGF-BB-induced intracellular LC3B expression (E, G) and mitochondrial autophagy (H, I) (magnification, 200×; scale bar, 20 μm). Western blotting indicated the downregulation of autophagy-related proteins ULK1, p-ULK1, Pink1, Parkin, P62, and LC3II (J, K). Glucose metabolism analysis demonstrated that miR-26a-5p inhibited PDGF-BB-induced glucose uptake (N) and lactate secretion (O) in hOMF cells, and Western blotting showed that miR-26a-5p inhibited PDGF-BB-induced expression of glucose metabolism-related proteins Glut-1, LDH-A, and MCT-4 (L-M)