Fig. 7

A 2/3 volume of CAF supernatant significantly promoted Cal-27 cell proliferation (A). CHC inhibited the proliferative effect of CAF supernatant (B). Lactic acid directly stimulated Cal-27 cells and promoted proliferation in a concentration-dependent manner (C). Western blotting that CAF supernatant upregulated the expression of NF-κB, p-NF-κB, HK2, Glut1, and LDHB proteins in Cal-27 cells (D, E), enhanced anti-apoptotic protein Bcl-2, and decreased pro-apoptotic protein Bax levels (H, K). CHC and SC75741 both inhibited the induction of NF-κB, p-NF-κB, MCT-1, LDH-B, HK2, and Glut1 protein (F, G) by lactate. qRT-PCR (P) and ELISA showed that lactate promoted the expression and secretion of PDGF-BB in Cal-27 cells, which was also inhibited by CHC and SC75741 (Q). After the knockdown of MCT1 gene expression in Cal-27 cells, CAF-derived lactate promoted diminished the migration (I, L) and invasion (J, N) abilities of tongue squamous carcinoma cells. In addition, the expression of NF-κB, p-NF-κB, PDGF-BB, HK2, Glut1, and LDHB proteins in Cal-27 cells was subsequently reduced (M, O)