Fig. 8
From: PDGF-BB accelerates TSCC via fibroblast lactates limiting miR-26a-5p and boosting mitophagy
Mixed xenografts were assessed before and after tumor removal (A). HE staining revealed a large number of mesenchymal components visible in the tumor tissues of each group (scale bars 400 μm; 100 μm) (B). Immunohistochemical staining demonstrated that α-SMA, MCT-4, and LDH-A were predominantly expressed in the mesenchyme, while p-NF-ĸB was mainly expressed in tumor cells (scale bar 400 μm; 100 μm) (C). PDGF-BB-induced CAFs were found to promote tumor volume (D) and weight (E). Multiplex immunofluorescence co-localization staining revealed significant co-localization of α-SMA, MCT-4, and LDH-A in the mesenchyme, which was most pronounced adjacent to tumor cells (scale bar 100 μm; 20 μm) (F)