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Fig. 3 | Cancer Cell International

Fig. 3

From: LncRNA AGAP2 antisense RNA 1 stabilized by insulin-like growth factor 2 mRNA binding protein 3 promotes macrophage M2 polarization in clear cell renal cell carcinoma through regulation of the microRNA-9-5p/THBS2/PI3K-Akt pathway

Fig. 3

AGAP2-AS1 binds to miR-9-5p to elevate THBS2 expression. A Venn diagram showing the overlap of LncBase database predicted AGAP2-AS1-bound miRNAs with lowly expressed miRNAs in the GSE37989 dataset. B Schematic diagram of the binding site between AGAP2-AS1 and miR-9-5p. C miR-9-5p expression in clinical samples. D, E Binding of AGAP2-AS1 and miR-9-5p assessed by dual luciferase reporter assay. F, G Expression of miR-9-5p in 786-O and ACHN cells after AGAP2-AS1 silencing or overexpression. H FISH analysis showing the co-localization of AGAP2-AS1 and miR-9-5p in 786-O and ACHN cells. I The expression of THBS2 in ccRCC in the GSE40435 dataset. J The expression of THBS2 in clinical samples. K Schematic diagram of the binding site between miR-9-5p and THBS2. L Binding of miR-9-5p and THBS2 assessed by dual luciferase reporter assay. M, N THBS2 expression in cells following miR-9-5p inhibitor/mimic treatment. O, P Expression of AGAP2-AS1, miR-9-5p and THBS2 in cells following miR-9-5p mimic and oe-AGAP2-AS1 treatment. Q THBS2 protein expression following miR-9-5p mimic and oe-AGAP2-AS1 treatment. * p < 0.05 vs. Normal group, mimic NC group, Bio-NC group, sh-NC group or mimic NC + oe-NC group. # p < 0.05 vs. Vector group or miR-9-5p mimic + oe-NC group

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Cancer Cell International

ISSN: 1475-2867

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