Fig. 1

CircFNDC3B and FNDC3B mRNA were dysregulated in OS. A Schematic diagram exhibiting the origination of FNDC3B mRNA and circFNDC3B. B Divergent and convergent primers amplifying circFNDC3B and FNDC3B mRNA in cDNA and gDNA. C The back-splicing junction site of circFNDC3B was validated by sanger sequencing. D, E CircFNDC3B and FNDC3B mRNA levels in OS cell lines and tissues were detected by qRT-PCR. F Correlation between circFNDC3B and FNDC3B mRNA levels in OS tissues was analyzed by Pearson method. (G) Overall survival of OS patients analyzed with the Kaplan-Meier method and log-rank test. Data were shown as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001