Fig. 4
The circFNDC3B-FNDC3B mRNA imbalance was a consequence of alternative splicing of RBM47. A, B Regulation of RBM47 on circFNDC3B and FNDC3B mRNA expression evaluated by qRT-PCR. C Predicted regulation pattern of RBM47 on circFNDC3B-FNDC3B mRNA imbalance. D Subcellular localization of RBM47 protein displayed by immunofluorescence; scale bar represents 5 μm. E Interaction of RBM47 with intron 1 and intron 2 sequences validated by RIP assays. F, G RBM47 mRNA levels in OS cell lines and tissues were detected by qRT-PCR. H Correlation of RBM47 expression with circFNDC3B or FNDC3B mRNA levels in OS tissues were analyzed by Pearson method. Data were shown as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001