Fig. 4

Co-injection of Hs738 overexpressing Serpin E1 and primary GC cells promotes peritoneal dissemination of cancer cells by promoting VEGFA expression and angiogenesis in nude mice. A Hs738 cells-derived Serpin E1 facilitates peritoneal dissemination of primary GC cells. Mice were killed 28 days after injection. B Quantitative analysis of tumor nodules in the peritoneal cavity by counting. C HE staining and IHC analysis of Ki67, Serpin E1, VEGFA, and CD31 on sections from the peritoneal tumor. Scale bars: 1 mm (left 2 columns) and 100 μm (right 3 columns). D Quantification of IHC staining using the Image J software (n = 9 tumor nodules per mouse). Bar graphs and dot plots show the mean ± SD, n = 5; **p < 0.05, and ***p < 0.001