Fig. 7

recSerpin E1 promotes the migration, tube formation, and p38 MAPK-dependent VEGFA expression in HUVECs. A recSerpin E promotes chemotactic migration of HUVECs by Matrigel-coated Transwell system. B, C VEGFA expression was determined in HUVECs treated with recSerpin El by immunofluorescence staining (B) and Western blotting (C). Scale bar = 10μm. D, E Tube formation on Matrigel (D) and VEGFA expression (E) were assessed in HUVECs co-treated with recSerpin E1 and anti-Serpin E1 antibody at a 6-h interval. Scale bar = 100μm. F Expression of p-p38, p-ERK1/2, p-JNK, and VEGFA was detected by Western blotting in HUVECs co-treated with recSerpin E1 (1 ng/ml) and anti-Serpin E1 antibody (2 μg/ml) or MAPK phosphorylation inhibitor (E)-osmundacetone (2 μM) at a 24-h interval. Bar graphs show the mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001