Fig. 1

lncRNA FOXD1-AS1 expression is up-regulated in pancreatic cancer CSCs. A Spearman’s correlation analysis was performed on RT-PCR data to investigate the relationship between lncRNA FOXD1-AS1 concentration and CD133 in primary PC cells (n = 30). The data, transformed to β-actin as △Ct, were analyzed to establish a correlation. B The association between lncRNA FOXD1-AS1 levels and CD90 in primary PC cells (n = 30) was examined using RT-PCR, and the data were normalized to β-actin as △Ct. Spearman’s correlation analysis was employed for statistical evaluation. C Real-time PCR was utilized to assess lncRNA FOXD1-AS1 expression in CD133 + and CD133- PC cells. D RT-PCR was employed to compare lncRNA FOXD1-AS1 expression in CD90 + and CD90- PC cells. E The expression of lncRNA FOXD1-AS1 in PC spheroids and adherent cells was evaluated through real-time PCR. F Serial passages of PC spheroids were subjected to RT-PCR to evaluate the expression of lncRNA FOXD1-AS1