Fig. 2

lncRNA FOXD1-AS1 knockdown inhibited pancreatic cancer CSC self-renewal and tumorigenesis. A MIA Paca-2 and AsPC-1 cells were infected with lncRNA FOXD1-AS1 knockdown virus, and the overexpression effect was verified by real-time PCR. B and C RT-PCR analysis was conducted to assess the expression levels of CD133 and CD90 in lncRNA FOXD1-AS1 knockdown and control PC cells. D The study utilized a spheroid formation experiment to investigate the effects of lncRNA FOXD1-AS1 knockdown on cellular behavior in PC cells, in contrast to control cells. Representative visuals depict the formation of spheres. E The percentage of pancreatic cancer CSCs in lncRNA FOXD1-AS1 knockdown cells and control PC cells was compared using an in vitro limiting dilution assay. F An in vivo limiting dilution assay was performed on lncRNA FOXD1-AS1 knockdown cells and control PC cells. Tumors were monitored over 2 months, with n = 5 for each group