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Fig. 3 | Cancer Cell International

Fig. 3

From: LncRNA FOXD1-AS1 regulates pancreatic cancer stem cell properties and 5-FU resistance by regulating the miR-570-3p/SPP1 axis as a ceRNA

Fig. 3

lncRNA FOXD1-AS1 overexpression promotes pancreatic cancer CSC self-renewal and tumorigenesis. A MIA Paca-2 and AsPC-1 cells were infected with a virus overexpressing lncRNA FOXD1-AS1, and the efficacy of overexpression was subsequently assessed using RT-PCR. B and C RT-PCR analysis was employed to evaluate CD133 and CD90 expression in control PC cells and lncRNA FOXD1-AS1 overexpressing cells. D The study utilized a spheroid formation assay to examine the impact of lncRNA FOXD1-AS1 overexpression on cellular behavior in PC cells, with representative images of spheres shown. E The percentage of pancreatic cancer CSCs in lncRNA FOXD1-AS1 overexpressing and control PC cells, compared using an in vitro limiting dilution assay. F An in vivo limiting dilution assay was conducted on lncRNA FOXD1-AS1 overexpressing and control PC cells. Tumors were monitored over 2 months with n = 5 for each group

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Cancer Cell International

ISSN: 1475-2867

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