Fig. 4

lncRNA FOXD1-AS1 enhanced SPP1 expression by sponging miR-570-3p as a ceRNA. A Heatmap illustrating differentially expressed genes in control and lncRNA FOXD1-AS1-KD PC cells. B Western blot analysis of SPP1 protein in lncRNA FOXD1-AS1 knockdown and PC control cells. C Venn diagram demonstrating the common complementary binding sites shared by miR-570-3p and miR-4272 in lncRNA FOXD1-AS1 and SPP1. D Bioinformatics analysis identified specific regions where miR-570-3p interacts with lncRNA FOXD1-AS1. E RT-PCR quantifying miR-570-3p expression. F Luciferase reporter assays conducted in specified PC cell lines. G The enrichment of lncRNA FOXD1-AS1 and microRNA miR-570-3p in Ago2-immunoprecipitates compared to the IgG control. H Bioinformatics analysis identified specific regions within the SPP1 gene targeted by miR-570-3p. I Luciferase reporter assays conducted on specified PC cell lines. J RT-qPCR analysis of SPP1 mRNA levels in the indicated PC cell lines. K Detection of miR-570-3p expression in PC cells by RT-qPCR following transfection with antagomir-NC or antagomiR-570-3p. L SPP1 mRNA levels in the PC cell lines were quantified through RT-qPCR. M RT-PCR was conducted to examine the relationship between the concentrations of lncRNA FOXD1-AS1, miR-570-3p, and SPP1 in primary PC cells (n = 30). The data were normalized using β-actin as △Ct and afterward analyzed using Spearman's correlation method