Fig. 5

lncRNA FOXD1-AS1 determines the response to 5-FU in PC cells. A Real-time PCR analysis of lncRNA FOXD1-AS1 5-FU-resistant and control PC cells. B PC cells with lncRNA FOXD1-AS1 knockdown or control were infected with SPP1 overexpression or a negative control virus for 48 h before being treated with 5-FU (10 μM). Flow cytometry was utilized to determine the proportion of apoptotic cells. C The Kaplan–Meier assay was employed to determine the overall survival of PC patients treated with 5-FU in the high lncRNA FOXD1-AS1 (n = 15) or low lncRNA FOXD1-AS1 (n = 15) groups. D PDXs with low lncRNA FOXD1-AS1 levels generated from primary PCs were treated for 24 days with 5-FU or saline (n = 3). The growth rate of the xenograft was determined. E Three groups of PDXs were included in this study. PDXs were derived from primary PCs exhibiting high levels of lncRNA FOXD1-AS1. These PDXs were then treated with either 5-FU or saline for 24 days. Each group consisted of three PDXs, and the rate of xenograft growth was determined