Fig. 6
m6A RNA methylation underlies the upregulation of FOXD1-AS1 in PC. A qRT-PCR was used to analyze the expression of lncRNA FOXD1-AS1 in the specified PC cell lines. B m6A levels in lncRNA-FOXD1-AS1 cells were determined using m6A-qPCR in PC cells transfected with si-NC or si-METTL3 #1, #2. C A RIP assay with anti-METTL3 antibodies was conducted to assess the interaction between METTL3 and lncRNA-FOXD1-AS1. D The SRAMP website was utilized to identify the specific m6A methylation locations of lncRNA-FOXD1-AS1. E In PC cells co-transfected with siMETTL3#1, #2, or siNC, the relative luciferase activity of pMIR-REPORT-LNCRNA-DBH-AS1 with either WT or MUT (A-to-T mutation) m6A sites was measured. Renilla luciferase activity was used as the standard to assess and normalize Firefly luciferase activity. F m6A-qPCR was employed to determine the m6-site levels in lncRNA-DBH-AS1 in PC cells transfected with siMETTL3#1, #2, or siNC. G Relative luciferase activity of pMIR-REPORT-lncRNA-FOXD1-AS1 co-transfected with siMETTL3#1, #2, or siNC in MIA Paca-2 and AsPC-1 cells with WT or MUT (A-to-T mutation) m6A sites, respectively. Renilla luciferase activity was used as the standard to assess and normalize Firefly luciferase activity. F lncRNA-FOXD1-AS1 levels in PC cells treated with actinomycin D (10 μM) were compared to ACTIN using qRT-PCR