Fig. 7
YTHDF1 improves lncRNA-FOXD1-AS1 mRNA stability in a m6A-dependent manner. A The binding of lncRNA-FOXD1-AS1 to the YTHDF1 protein in PC was confirmed by RIP-qPCR. B YTHDF1 was assessed via Western blotting in PC using cell lysate, full-length biotinylated lncRNA-FOXD1-AS1, and only beads (NC). C Western blot analysis of YTHDF1 in PC with cell lysate (Ly.), completely biotinylated-lncRNA-FOXD1-AS1 (#1), lncRNA-FOXD1-AS1(0–400) with m6A pattern site 1 and 2-WT (#2) and site 1 and 2-MUT (#3), lncRNA-FOXD1-AS1(400–600) with m6A pattern site 3-WT (#4) and site 3-MUT (#5), and beads alone (NC). D Analysis of lncRNA-FOXD1-AS1 levels with ACTIN using qRT-PCR in PC cells treated with actinomycin D (10 μM)