Fig. 2

KCNA1 knockdown inhibits GBM proliferation and invasion. A Western blot to verify KCNA1 knockdown efficiency, B Immunofluorescence to verify KCNA1 knockdown efficiency (the scale is bar 100 μm). C CCK-8 assay for cell viability of SHG140 and U87 cells after transfection (n = 3), D Colony formation assays of SHG140 and U87 cells to measure the proliferation capacity (n = 3). E Western blot to detect expression of MMP2, MMP9, N-Cadherin and Snail in SHG140 and U87 after transfection, F Transwell assay of SHG140 and U87 cells after transfection (Scale bar = 500 μm), G 3D tumor spheroid invasion assay to assess invasion of SHG140 and U87 cells (Scale bar = 500 μm). One-way ANOVA for multi-group comparisons. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. GBM, glioblastoma; shKCNA1, KCNA1- shorthair pin RNA