Fig. 3

KCNA1 promotes GBM progression in vitro. A Western blot to verify KCNA1 overexpress efficiency, B Immunofluorescence to verify KCNA1 overexpress efficiency (the scale is bar 100 μm). C CCK-8 assay for cell viability of SHG140 and U87 cells after transfection (n = 3), D Colony formation assays of SHG140 and U87 cells to measure the proliferation capacity (n = 3). E Western blot to detect expression of MMP2, MMP9, N-Cadherin and Snail in SHG140 and U87 cells after transfection, F scratch assay of SHG140 and U87 cells after transfection (Scale bar = 1 mm), G Organoids assay transfected with NC and KCNA1-OE (Scale bar = 500 μm). Student’s t-test for two-group comparison. n = 3, **p < 0.01, ***p < 0.001, ****p < 0.0001