Fig. 5

Knockdown of KCNA1 promotes ferroptosis. A Expression of ferroptosis-associated proteins cells after transfection, B ATP produced by U87 and SHG140 cells was detected using an enhanced ATP assay kit, C The GSH in U87 and SHG140 cells was detected using a GSSG/GSH assay kit, D Transmission electron microscopy in U87 and SHG140 cells. E Mitochondrial membrane potential was detected using a JC-1 assay kit, F–G Intracellular ROS level was detected using a DCFH-DA assay kit, H–I Intramitochondrial ROS level was detected using a MitoSOX assay kit, J–K The accumulation of lipid ROS was detected using a C11-BODIPY 581/591 assay kit. One-way ANOVA for multi-group comparisons. n = 3, **p < 0.01, ***p < 0.001, ****p < 0.0001